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In four of these cases, BRAF mutation and RET/PTC rearrangement were coexisting. The BRAF mutation was underrepresented among PTCs with accompanying chronic lymphocytic thyroiditis (CLT) compared with PTCs without this feature (12 vs 44%).

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In four of these cases, BRAF mutation and RET/PTC rearrangement were coexisting. The BRAF mutation was underrepresented among PTCs with accompanying chronic lymphocytic thyroiditis (CLT) compared with PTCs without this feature (12 vs 44%). Comparison of 10 human cytokines and chemokines measured in humanized mouse serum that were (i) infected by mosquito bite (black bars), (ii) bitten by uninfected mosquitoes (white bars), and (iii) infected by virus injection (hatched), the…

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Transfection was performed using Lipofectamine PLUS (Invitrogen) according to the protocol of the manufacturer. After being cultured for 24 h, cells were subjected to Western blotting as described above.

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